Fig. 3.
Flow cytometry on bone marrow, splenocytes and blood mononuclear cells from mice infused with 100 × 104▵MGMT-transduced cells after 1, 2, or 3 cycles of BG and BCNU.
Cells were fixed, permeabilized, and stained with the human AGT-specific monoclonal antibody mT3.1 and a PE-conjugated secondary antibody before analysis. There was no cross-reactivity with murine AGT. The proportion of cells overexpressing δAGT was determined by subtracting the histogram obtained from normal mouse tissue.
