American Society of Hematology

Fig. 4.

$Fig. 4. TcdB-10463–mediated inhibition of LPS-induced IL-8 secretion is not due to intracellular accumulation of IL-8. / Cells were incubated with 10 ng/ml TcdB-10463 for 1 hour prior to LPS stimulation; 100 ng/mL LPS was added for 8 hours as indicated. Supernatant was collected, and cells were washed three times, permeabilized with 100 ng/mL saponin, and resuspended. Supernatants and cell extracts representing the intracellular fraction were subjected to ELISA analysis. Data presented are mean ± SEM of 3 separate experiments.$

TcdB-10463–mediated inhibition of LPS-induced IL-8 secretion is not due to intracellular accumulation of IL-8.

Cells were incubated with 10 ng/ml TcdB-10463 for 1 hour prior to LPS stimulation; 100 ng/mL LPS was added for 8 hours as indicated. Supernatant was collected, and cells were washed three times, permeabilized with 100 ng/mL saponin, and resuspended. Supernatants and cell extracts representing the intracellular fraction were subjected to ELISA analysis. Data presented are mean ± SEM of 3 separate experiments.

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