Recognition of rvWF by mAbs, of which the previously identified epitope is mentioned in parentheses.

Conditioned media samples diluted to approximately 0.01 U/mL vWF:Ag were incubated for 1 hour at 37°C into a microtiter plate coated with each mAb. After washing, the bound rvWF was detected with peroxidase-conjugated anti-vWF polyclonal antibodies. Absorbance at 492 nm was converted to milliunits of vWF:Ag using standard curves generated for each capture mAb using dilutions of a calibrated pool of normal plasmas. Each sample of rvWF was tested in triplicate, and data are illustrated as mean ± SD.