RT-PCR analysis of Ly-6M expression.

The control β-actin signal (270 bp) is the upper band and the Ly-6M signal (149 bp) is the lower band. The templates are as follows: lane 1 (spleen, no RT), lane 2 (peritoneal macrophages), lane 3 (spleen), lane 4 (thymus), lane 5 (bone marrow), lane 6 (liver), lane 7 (lung), lane 8 (heart), lane 9 (kidney), lane 10 (stomach), lane 11 (small intestine), lane 12 (skin), and lane 13 (brain). PCR products were resolved on 8% polyacrylamide and visualized by autoradiography after a 4-hour exposure.