Fig. 3.
Histograms from measurement of P-selectin expression by laser scanning cytometry
to illustrate the level of P-selectin expression in HUVEC after incubation with medium (A) or IL-13 (50 ng/mL) (B) for 48 hours. Further rapid up-regulation occurs after stimulation with histamine (10−5 mol/L) for 10 minutes (C). Histograms show mean fluorescent values detected within each cell perimeter using anti-P–selectin or isotype control (outline) antibody.
