Fig. 2.
Priming in the presence of mAbs induces hyporesponsiveness.
Primary MLCs were performed for 7 days either in the absence (control) or presence of mAbs. Primed cells (2 × 104) were restimulated with the original allogeneic stimulator PBMCs (1 × 105). (A) Proliferative response at day 3 of culture by 3H incorporation. (B) IFN-γ and IL-2 production were analyzed in the culture supernatants. (C) Proliferation kinetics of secondary response of control and hyporesponsive T cells. Results are expressed as mean and SE of quadruplicate (A, C) and duplicate (B) measurements. Representative experiments are shown.
