Cell cycle–related protein levels.

Western blot analysis was performed for c-myc, bcl-xL, bcl-2, bax, p16, p21, and p27 protein levels in 72-hour cultured CLRPP in the presence (anti–TGF-beta1) or the absence (Ctrl CLRPP) of 10 μg/mL anti-TGF-β1 neutralizing antibody. Bar graph shows the mean ± SD relative adsorbance ratio between the protein of interest and actin observed in 4 different experiments. Parallel cultures in the presence of irrelevant isotype-matched mouse immunoglobulins showed comparable results to those observed in control CLRPP cultures. P > .05 for c-myc, bcl-xL, bax, p16, and p21 protein levels of control and study CLRPP; P < .01 for bcl-2 and P < .0001 for p27 protein levels.