CLRPP growth and CFU-GM/BFU-E content.

CLRPP growth (upper graph) and CLRPP-derived CFU-GM (center graph) and BFU-E (lower graph) contents were determined after 72 hours of culture in serum-free conditions in the presence or the absence of several doses of anti-TGF-β1 neutralizing mAb. P > .05 for any anti-TGF-β1 dose on CLRPP growth. P < .0001 for an anti-TGF-β1 dose ≥ 1 μg/mL on CLRPP-derived CFU-GM and BFU-E content. Parallel cultures in the presence of irrelevant isotype-matched mouse immunoglobulins showed comparable results to those observed in control CLRPP cultures. Results are expressed as the mean values ± SD observed in 4 different experiments. In each graph, • indicates the control; ▾, anti-TGF-β 0.1 μg/mL; □, anti-TGF-β 1 μg/mL; +, anti-TGF-β 10 μg/mL; ▪, anti-TGF-β 20 μg/mL.