Methylation maps of E-cad gene CpG island.

(A) Summary of the direct PCR sequencing analysis for normal and leukemic samples. The sample number and leukemia subtype (FAB classification) are in the left column, and the CpG sites are numbered across the top row. ◍ represents up to 25% methylation, ◍ represents 26% to 50% methylation, ◍ represents 51% to 75% methylation, • represents 76% to 100% methylation, and ○ represents no methylation, as determined by direct PCR sequencing. Absence of a score indicates that the methylation at that site was unable to be determined due to enzyme stoppage in the sequence. (B) Methylation results for CD34⁺ cells. The top row represents the direct sequencing results, followed by the methylation patterns for 10 cloned molecules. + indicates a methylated CpG site and − indicates an unmethylated CpG site.