Map of the E-cad gene.

(A) CpG island plot across the 5′ promoter region through to exon 2 (compiled from Genbank accession numbers L34545, L36526, and L34937). The dark line is the %G+C, and the lighter line is the observed/expected CpG density (O/E CpG). (B) Schematic representation of the gene, where the black boxes represent the coding regions of exon 1 and exon 2. (C) The E-cad gene DNA sequence analyzed by bisulphite genomic sequencing from bases 863-1138 (L34545), incorporating 29 CpG sites. CpG dinucleotides are numbered below each site. The Hpa II sites are underlined with a solid line, and the GC region is underlined with a dotted line. Other features are boxed and labeled. An arrow represents the transcription start site between CpG sites 15 and 16. The identified polymorphism (A or T), after bisulphite sequencing, is at coordinate 862, and is immediately 5′ to CpG site 1. Bisulphite sequencing of this region identified an additional thymine at *, which is not present in the published sequence (L34545).