Expression of human Aγ-globin mRNA in transgenic mice containing the BGT35 construct.

S1 nuclease analysis on fetal liver RNA of 15.5-day founder transgenic mice showing BGT35 is expressed at significant levels in 5 of the 7 transgenic mice. These data show that the β-globin promoter element and intron 2 sequence are not sufficient for reproducible single-copy transgene expression. Abbreviations as described in Figure 3.