Thrombin induction of apoptosis demonstrated by Annexin V assay.

PAR-1–transfected B16F10 (S14) cells were incubated without thrombin or with thrombin: 0.25 and 1.5 U/mL for 9, 19, and 24 hours and the degree of Annexin V and propidium iodide binding determined by flow cytometry. Annexin V binds to early apoptotis and late apoptotic/necrotic cells. Propidium iodide binds to late apoptotic/necrotic cells. Percentage of cells in the upper and lower right quadrants is given.