Increased sensitivity of PAR-1–transfected HCT8 tumor cells to thrombin-induced impaired cell growth.

(A) Immunoblot of PAR-1 transfected cells with PAR-1–tagged anti-FLAG antibody. Lane 1, wild-type cells; lane 2, empty plasmid-transfected cells; lane 3, wild-type PAR-1 cells. (B) Wild-type PAR-1 transfectants were incubated with various concentrations of thrombin for 2 days. Control bars (black) depict empty vector transfection, whereas gray bars depict PAR-1 transfection. SEM is given for experiments performed in quadruplicate.