Absence of PAR-4 in B16F10 cells.

Lane M (mw markers), lanes 1 to 3 (Actin), lanes 4 to 6 (PAR-4), lanes 1 and 4 (B16F10 cells), lanes 2 and 5 (water blank), lanes 3 and 6 (mouse spleen cells). Similar negative results for PAR-4 was obtained with HCT8 cells.