Changes in TPO mRNA expression in human BM stromal cells measured by semiquantitative reverse transcriptase (RT)-PCR.

Semiquantitative RT-PCR analyses used RNA isolated from primary BM stromal cells. The PCR products were separated by agarose-gel electrophoresis and analyzed by Southern hybridization with a gene-specific probe. It was confirmed that TPO mRNA expression was up-regulated after 4 hours of incubation with 50 ng/mL PDGF (A) or 20 ng/mL FGF (B) and down-regulated with 1 μg/mL PF4 (C), 1 μg/mL TSP (D), and 50 ng/mL TGF-β.