Fig. 6.
Fig. 6. Optimization of quantitative polymerase chain reaction (PCR) analysis. / Autoradiograph showing PCR carried out with an increasing number of cycles and with the products subjected to Southern hybridization with a gene-specific probe. The optimal cycle number was determined to be in the midlinear region of the curve: 31 cycles for TPO transcripts (A) and 18 cycles for GAPDH (B).

Optimization of quantitative polymerase chain reaction (PCR) analysis.

Autoradiograph showing PCR carried out with an increasing number of cycles and with the products subjected to Southern hybridization with a gene-specific probe. The optimal cycle number was determined to be in the midlinear region of the curve: 31 cycles for TPO transcripts (A) and 18 cycles for GAPDH (B).

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