Stability of lymphoid reconstitution in γ_c-transduced mice.

Flow cytometric analysis of peripheral blood T and B cells using FITC-conjugated anti-TCRαβ and PE-conjugated anti-B220 antibodies. Positive cells are expressed as percentage of gated lymphocytes. The left panel demonstrates long-term peripheral reconstitution 47 weeks posttransfer with normal percentage of T and B cells. The center panel shows a γ_c-transduced animal at 8 weeks postgraft. Bone marrow cells from this mouse were transferred into a secondary irradiated recipient, which, after 8 weeks, demonstrated normal peripheral reconstitution (right panel).