Characterization of splenic and gut lymphocytes from γc-transduced mice.
Flow cytometric analysis of splenocytes using (A) FITC-conjugated anti-CD8 and PE-conjugated anti-CD4 antibodies or (B) FITC-conjugated anti-immunoglobulin D (IgD) and PE-conjugated anti-IgM antibodies. (C) Analysis of hepatic lymphocytes using FITC-conjugated anti-DX5 (natural killer cell specific) and PE-conjugated anti-TCRαβ antibodies. Positive cells are expressed as percentage of gated lymphocytes. (D) Representative sections of small bowel epithelium in a γc-transduced mouse (Periodic Acid Shiff staining, × 250). Intraepithelial lymphocytes (arrows), normal cellularity of the lamina propria, and lymphoid cryptopatches (large arrow) were identified that were indistinguishable from control γc+ animals16 (and data not shown).
