Fig. 3.
Pretreatment with DEX does not affect the regulation of DC-antigen uptake machinery.
Immature DCs were incubated in the absence or the presence of 10−6 mol/L DEX for 24 hours and further activated or not via CD40 with the CD8-CD40L fusion protein for 48 hours. Cells were pulsed for 1 hour with medium containing either 1 mg/mL FITC-BSA or 1 mg/mL FITC-mannosylated BSA. Empty histograms show the background autofluorescence, gray-filled histograms show the background uptake at 0°C, and black-filled histograms show the specific uptake at 37°C. Data are representative of 3 independent experiments.
