Fig. 1.
Pretreatment with DEX inhibits the phenotypic changes induced by CD40 ligation.
Seven-day immature DCs were cultured for 24 hours in the absence or the presence of 10−6 mol/L DEX and activated via CD40 with the CD8-CD40L fusion protein for 48 hours. The comparison with immature DCs maintained in medium alone is shown. Empty histograms show the background staining with isotype controls monoclonal antibody, and solid histograms represent specific staining of the indicated cell-surface markers. Specific mean fluorescence intensities are indicated. Mean fluorescence intensities of isotype controls were between 3 and 4. Data are representative of 4 independent experiments.
