Induction of MEFV expression during granulopoiesis ex vivo.

(A) Wright-Giemsa–stained cytospin preparations of cells from PBHP cultures induced to differentiate toward the granulocytic lineage. Representative images from cultures at 3, 7, 10, 13, 17, and 21 days are shown. Original magnification ×600. (B) EtBr-stained PAGE of RT-PCR products of the MEFV, lactoferrin (LF), myeloperoxidase (MPO), and β-actin mRNAs from PBHP cells collected on the days indicated. Positive and negative control PCR reactions using the MEFV-containing plasmid pV75-1 and water, respectively, are shown.