Amplification of the 3q27 region as detected with the D3S1311 microsatellite marker and confirmatory CGH for chromosome 3 in patient 22.

Control and tumor DNA samples were amplified with markers D3S1311 located in the 3q27 region and D7S496 used as an internal control in a multiplex PCR reaction, and the ratios of the alleles determined. CGH profile of chromosome 3 of the same patient is shown on the right side. The cut-off values for losses (0.80) and gains (1.25) are depicted as blue and magenta lines, respectively.