PKC- and PKC-δ inhibit IL-3–induced cellular tyrosine phosphorylation.

32D-wt, 32D-αb 32D-δ, and 32D-ε cells were starved for 18 hours (0.5% FCS), pretreated with TPA (100 ng/mL) for the indicated times, and stimulated with IL-3 (10 ng/mL) for 15 minutes. 25 μg total cellular protein was loaded on each lane, and tyrosine phosphorylation was detected by immunoblotting.