Circulating ICAM-1 concentrations in serum from LPS-treated mice.

Mice were given a single dose of 40 mg/kg of LPS intraperitoneally. Blood samples were taken from the retro-orbital plexus with heparinized capillaries before (0 hour) and at indicated times after LPS injection. At different time points, different groups of animals were used. Serum ICAM-1 concentrations were analyzed by ELISA-2 in duplicate. Results are expressed as the mean (± SEM) ICAM-1 immunoreactivity.