American Society of Hematology

Fig. 4.

$Fig. 4. Comparison of cICAM-1 in wild-type and mutant C57BL/6 mice by fast protein liquid size exclusion chromatography. / Sterile filtered serum (500 μL) from wild-type (+/+), ICAM-1–exon 4 mutant mice (-/-) and a mixture of +/+ and -/-serum were chromatographed on a Superdex-200 column in separate runs. Collected fractions of 0.5 mL were analyzed for the presence of cICAM-1 by ELISA-1. The molecular weight of proteins in the different fractions was estimated from the elution profile of marker proteins of molecular weight in the range of 12 to 200 kd.$

Comparison of cICAM-1 in wild-type and mutant C57BL/6 mice by fast protein liquid size exclusion chromatography.

Sterile filtered serum (500 μL) from wild-type (+/+), ICAM-1–exon 4 mutant mice (-/-) and a mixture of +/+ and -/-serum were chromatographed on a Superdex-200 column in separate runs. Collected fractions of 0.5 mL were analyzed for the presence of cICAM-1 by ELISA-1. The molecular weight of proteins in the different fractions was estimated from the elution profile of marker proteins of molecular weight in the range of 12 to 200 kd.

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