American Society of Hematology

Fig. 6.

$Fig. 6. Determination of BCR/ABL mRNA in LAMA84 and LAMA84R cells. / Samples containing 25 μg of total RNA per lane, extracted from LAMA84 and LAMA84R, were fractionated and analyzed by Northern blotting. A highly specific 40mer, corresponding to a portion of the b3-a2 junction sequence of BCR/ABL,14 was used as a probe and detected an 85-Kb transcript (upper panel). The same membrane was rehybridized with a murine β-actin probe (lower panel).$

Determination of BCR/ABL mRNA in LAMA84 and LAMA84R cells.

Samples containing 25 μg of total RNA per lane, extracted from LAMA84 and LAMA84R, were fractionated and analyzed by Northern blotting. A highly specific 40mer, corresponding to a portion of the b3-a2 junction sequence of BCR/ABL,14 was used as a probe and detected an 85-Kb transcript (upper panel). The same membrane was rehybridized with a murine β-actin probe (lower panel).

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