Double staining by anti-CD41a and CD42a MoAbs of CD34⁺ cells cultured for 8 and 15 days in liquid culture medium.

Blood CD34⁺ cells from a TAR syndrome patient were grown in liquid medium in the presence of SCF plus PEG-rHuMGDF or a combination of 3 cytokines (IL-3, SCF, and IL-6). At days 8 and 15, cells were labeled by an R-PE anti-CD41a MoAb and FITC anti-CD42a MoAb and analyzed by flow cytometry. This figure illustrates the results obtained in 1 patient. Similar results were obtained in the 4 other patients studied.