Effects of PEG-rHuMGDF in liquid culture medium on the growth of MKs from CD34⁺ cells of TAR syndrome patients.

PEG-rHuMGDF was tested at different concentrations, alone or in combination with SCF. CD34⁺ cells (2000 cells) from bone marrow of a TAR syndrome patient were grown in a 96-well plate (100 μL) in triplicate. At day 10, the numbers of viable cells and viable CD41⁺ cells were quantitated by flow cytometry using staining with an R-PE anti-CD41a MoAb and 7AAD.