Delta-like-1 protein expression.

(A) Western blot with chicken isotype IgY control. No signal was observed at the position of 79 kd. (B,C) Western blot with affinity-purified anti-mDll1^NDSL antibody. mDll1 protein (79 kd was detected in adult mouse tissues, including lung, thymus, small intestine, brain, spleen, liver, and bone marrow. hDll1 protein (79 kd was also detected with the same antibody in human umbilical vein endothelial cells (HUVEC), HUVEC treated with IFN-αb and IL-1β (IL-1). (D) In vitro transcription and translation of thehDll1 cDNA. The full-length hDll1 cDNA was in vitro translated into a 79-kd protein. Autoradiograph of the³⁵S-labeled protein product separated by 10% SDS-PAGE was shown.