Comparison of the antigenic recovery of conventionally purified granzyme A (GrA) versus immunoaffinity purified GrA.

Detection by antigenic enzyme-linked immunosorbent assay of 18 ng conventionally purified GrA (A) and 18 ng GrA present in a degranulate (B) after incubation with 5 μL EDTA plasma for 30 minutes at 20°C.