![Fig. 7. Changes in cytosolic calcium in undifferentiated HL-60 cells transfected with either the FPR or the lipoxin A4receptor (LXA4R/FPRL1). / Undifferentiated HL-60 cells expressing either FPR or LXA4R/FPRL1 were loaded with 2 μmol Fura-2 and analyzed with respect to the rise in intracellular [Ca++] mediated by the application of peptides. (A) FPR-expressing HL-60 cells have a resting value [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 150 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−7 mol/L; open circles) or WKYMVm (final concentration, 10−7 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. (B) HL-60 cells expressing LXA4R/FPRL1 have a resting [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 300 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−6 mol/L; open circles) or WKYMVm (final concentration, 10−9 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. For both figures, abscissa, time of study; ordinate, concentration of [Ca++]i.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/5/10.1182_blood.v95.5.1810.005k06_1810_1818/6/bloo00506007ax.jpeg?Expires=1724137381&Signature=Fl3fII2KWpueTLvQf9zncPdN9khbXrgOhuANXAdt6dPCjRJyC7mroMPhMg-NEsQyEjCv1wgk1AS5gsLOVfSj--WP-foRq-kzLBL8UFryzmw~3uQQ74UtZ~OPzRTVrLdGPpepElYuFiSK89~tAFBNkhzANb7l4YH9P3wws3G5BESiWWV7VKfwg~74PVhF8aAHcR3k7G7wZfioqBbHfqXjcOtF4oiELpYwlyyjfH7LXn-ZrJgacQsPnrjUcWioPFpiOgyrzdRrGe9YymqbAXQ2IaeG4ecb9n76jjqWeAjoTZOpqkwPxhqxdSzR7DUk4J~RrNoy9Sa87kEbjz2I04Cbvg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Changes in cytosolic calcium in undifferentiated HL-60 cells transfected with either the FPR or the lipoxin A4receptor (LXA4R/FPRL1).
Undifferentiated HL-60 cells expressing either FPR or LXA4R/FPRL1 were loaded with 2 μmol Fura-2 and analyzed with respect to the rise in intracellular [Ca++] mediated by the application of peptides. (A) FPR-expressing HL-60 cells have a resting value [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 150 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−7 mol/L; open circles) or WKYMVm (final concentration, 10−7 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. (B) HL-60 cells expressing LXA4R/FPRL1 have a resting [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 300 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−6 mol/L; open circles) or WKYMVm (final concentration, 10−9 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. For both figures, abscissa, time of study; ordinate, concentration of [Ca++]i.
