Fig. 6.
RT-PCR analysis of LTC4 synthase mRNA expression in CML CD16+ neutrophils.
Total RNA was extracted from CML CD16+ neutrophils and normal CD16− eosinophil-enriched cell preparations and used as templates in RT-PCR reactions using LTC4synthase or β-actin specific primers. M represents a 50-2000 bp molecular weight marker. The expected lengths of amplification products are 119 bp (LTC4 synthase) and 784 bp (β-actin). One representative experiment out of 4 is shown.
