Fig. 7.
Inhibition of idiotype-specific cytotoxicity with specific antibodies.
Autologous DC were repetitively stimulated against the idiotype of autologous lymphoma cells with Fab-loaded dendritic cells. Specific cytotoxicity was assessed in a standard 51Cr release assay with idiotype heavy or light chain expressing autologous LCL as target cells. For the inhibition of lytic activity, effector cells were preincubated with anti-CD3, anti-CD4, or anti-CD8 antibody, and target cells were preincubated with anti-MHC class I or anti-MHC class II antibodies. AlloLCL, allogeneic LCL included as a negative control target.
