Effect of neutralizing antibodies against TNF- on PF4- and TNF--mediated rescue from monocyte apoptosis.

Monocytes were cultured for 72 hours in medium alone (left panel), with 4 μmol/L PF4 (middle panel), or with 10 ng/mL TNF-α (right panel) in the presence (dark columns) or absence (white columns) of neutralizing antibodies directed against TNF-α. Cells were subsequently labeled with annexin-V and PI, and the percentage of apoptotic cells was determined by flow cytometry. Data from 1 representative experiment out of 6 are shown (n.d. = not determined).