Support of splenocyte hematopoiesis by coculture with infected 14M1.4 cells is dependent on GM-CSF and TNF-.

14M1.4 cells were untreated (open symbols) or infected with LV9 amastigotes at a ratio of 25:1 (closed symbols). Adherent 14M1.4 cells were preincubated with (A) control HIgG (squares) or anti-GM-CSF mAb (circles), (B) control HIgG (squares) or anti-TNF-α mAb (circles) or (C) control GIgG (circles) or anti-MIP-1α Ab (squares) for 30 minutes before overlaying with naı̈ve splenocytes suspended in methylcellulose, in the absence of growth factors (Methocult 3230), but in the presence of hemin.