Fig. 3. Stromal macrophages do not affect hematopoietic colony formation in the presence of exogenous growth factors. / 14M1.4 cells were either untreated (open circles) or infected with amastigotes at a ratio of 25:1 (closed circles), and were overlaid with spleen (A and B) or bone marrow (C-E) cells suspended in methylcellulose containing PWM-SCCM, Epo, and SCF. After 7 days, mature colonies were scored as CFU-GM (A and C), BFU-E (B and D) and CFU-GEMM (E). Data represent the mean ± SEM for triplicate wells and are representative of 2 independent experiments.
Fig. 3.

Stromal macrophages do not affect hematopoietic colony formation in the presence of exogenous growth factors.

14M1.4 cells were either untreated (open circles) or infected with amastigotes at a ratio of 25:1 (closed circles), and were overlaid with spleen (A and B) or bone marrow (C-E) cells suspended in methylcellulose containing PWM-SCCM, Epo, and SCF. After 7 days, mature colonies were scored as CFU-GM (A and C), BFU-E (B and D) and CFU-GEMM (E). Data represent the mean ± SEM for triplicate wells and are representative of 2 independent experiments.

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