Annexin-V binding to thiazole orange-positive and -negative platelets stimulated with thrombin plus convulxin.

Platelets were stimulated with 5 nmol/L thrombin plus 500 ng/mL convulxin and then stained with PE–annexin-V to label negatively charged surface phospholipids and thiazole orange (TO) to identify reticulated platelets. Panel A represents the TO⁺platelets, Panel B depicts the entire population, and Panel C represents the TO⁻ platelets.