Characterization of anti-FV monoclonal antibodies.

Purified FV (solid line), FVa (shaded), and no protein (dotted) were added to phospholipid-coated beads as described in “Materials and methods.” FITC-labeled antibody against FV light chain (anti-HFV-237; Panel A), FV heavy chain (anti-HFV-271; Panel B), and FV connecting region (anti-HFV-227; Panel C) were then added and particle-bound fluorescence measured by flow cytometry.