Immunolocalization of CEP110 during the cell cycle.

HeLa cells were double-stained for CEP110 (C, F, I, L, O) and pericentrin (B, E, H, K, N) and counterstained for DNA with DAPI (A, D, G, J, M). In early interphase, CEP110 is seen as a small focus at the centrosome (C). In later interphase cells, when duplicated centrosomes begin to separate CEP110 reactivity, is only found at 1 centrosome (F, arrowhead). In late interphase–early prophase cells CEP110 reactivity can be found on both centrosomes (I). Pericentrin reactivity is prominent at the spindle poles during metaphase (K), but CEP110 reactivity is barely detectable (L). At telophase, CEP110 reactivity is detected as small foci at the centrosome in the daughter cells (O, arrowheads). Bar = 10 μm.