Bcl-2 expression.

A. Bcl-2 expression at day 0 and day 5. Right panel: cultures with recombinant thioredoxin (rTrx) 1 μg/mL; left panel: cultures without rTrx. B-type chronic lymphocytic leukemia (B-CLL) cells were obtained from patient RD-1. B. Bcl-2 expression 12 days after onset of cultures with Trx (Trx day 12, thick line) and without Trx (day 12, dotted line) in comparison with Bcl-2 expression at the onset of experiment (day 0, thin line). Flow cytometry analysis was performed as described in Materials and methods section. C = control mouse isotype IgG; counts = cell number; FL1-H = fluorescence intensity.