Fig. 4.
RT-PCR analysis of the XG transcripts from B-LCLs and somatic hybrid derivatives.
Total-cell RNA from the indicated cell lines was reverse transcribed and PCR-amplified to obtain a fragment of 551 bp corresponding to the coding sequence of the XG cDNA. The PCR products were electrophoresed on a 1.2% (wt/vol) agarose gel, transferred to a nylon membrane (Hybond N+; Amersham), and hybridized with a32P-labeled internal oligonucleotide probe. C, control PCR without template cDNA. The size (bp) of the PCR products was checked with EcoRI–HindIII-digested λ DNA markers.
