Fig. 7.
Detection of expression of Iya epitope on recombinant GP Ib-Ibβ-IX complexes by flow cytometry.
CHO cells stably expressing GP Ibα and GP IX were transiently transfected with eucaryotic expression vectors carrying wild-type (A) or mutated (B) complementary DNA for GPIbβ. Forty-eight hours after transfection, expression of the Iya epitope on the cell surface was determined by using anti-Iya antibodies (dark). Normal human serum was used as a negative control (bright). The arrow indicates the subpopulation of cells showing antibody binding.
