Fig. 3.
Immunoblotting studies.

Immunoblotting studies.

Aliquots of 109 washed platelets from an Iya-positive individual were lysed, and proteins were separated by using 7.5% SDS-PAGE under nonreducing (n.r.) and reducing (r.) conditions. After proteins were transferred to a nitrocellulose membrane, membrane strips were incubated with anti-Iyaantiserum (lane 1), MAB SZ2 (lane 2), and MAB Gi27 (lane 3). Antibody binding was detected by using corresponding peroxidase-conjugated antibodies and chemiluminescence substrate.

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