Fig. 2.
Immunoprecipitation analysis of the GPIb-Ibβ-IX complex of Iya-phenotyped platelets after trypsin treatment.

Immunoprecipitation analysis of the GPIb-Ibβ-IX complex of Iya-phenotyped platelets after trypsin treatment.

Aliquots of 109 biotinylated platelets from an Iya-positive individual were treated with trypsin, washed, lysed, and immunoprecipitated with MAB SZ2 (lane 1), MAB Gi10 (lane 2), MAB Gi27 (lane 3), and anti-Iya antibodies (lane 4). Immunoprecipitates were analyzed with 7.5% SDS-PAGE under nonreducing conditions.

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