Fig. 5.
Cleavage of PAR-1 peptide sequences.
(A) The peptide LDPRSFLLRNPNDKYEPF (TR18), corresponding to the uncleaved receptor, elutes as a single peak at 18 minutes. (B) The peptide SFLLRNPNDKYEPF (TR14), corresponding to the receptor following cleavage at the site resulting in receptor activation, elutes as a single peak at 5 minutes. (C) TR18 following 15 minutes incubation with 10 nM thrombin. (D) TR18 following 4 hours incubation with 0.5 μM Sk-plasminogen. (E) TR18 following 4 hours incubation with 0.5 μM Sk-plasminogen and 2 μM α2-antiplasmin. Significant peptide cleavage occurs even in the presence of α2-antiplasmin.
