Kinetics of G-CSF–induced Akt phosphorylation in BAF3 cells expressing the different forms of the G-CSF receptor.

(A) Schematic diagram of the wild-type (WT) and truncated forms of the G-CSF receptor. Boxes B1, B2, and B3 denote subdomains conserved in several members of the cytokine receptor superfamily. The numbers in parentheses indicate amino acid positions; TM, transmembrane domain. (B) Akt phosphorylation induced by G-CSF in BAF3 cells expressing the different G-CSF receptor forms. Cells were left untreated or treated with G-CSF for the indicated times. Whole-cell extracts were immunoblotted with anti-phospho-Akt antibody (upper panel) and reprobed with anti-Akt antibody (lower panel). (C) G-CSF stimulated phosphorylation of Akt in myeloid 32D cells expressing the wild type or the D715 form of the receptor.