Shear stress requirement for adhesion of flowing selectin-expressing cells to adherent HL-60 cells.

HL-60 cells were prebound to an antihuman CD43 mAb-coated glass slide. Pre-B L1-2 cells expressing P-, E-, or L-selectin were withdrawn over the prebound HL-60 cells. The number of transient cell-cell interactions was counted and normalized for the number of prebound HL-60 cells, the duration of the experiment, and the flux of cells passing through the system. The data are the average of 2 independent experiments.