American Society of Hematology

Fig. 1.

$Fig. 1. Purification and characterization of vMIP-III expressed in CHO cells. / (A) vMIP-III was purified from culture supernatants of stably transfected CHO cells by cation exchange chromatography. Column washes were performed at 0.2 mol/L NaCl/20 mmol/L Tris, pH 7.2, and vMIP-III was eluted at 0.8 mol/L NaCl/20 mmol/L Tris. (B) SDS-PAGE analysis followed by Coomassie blue staining of the peak fractions shown in A. (C) vMIP-III was subjected to MALDI-MS. The 9677.08 and 9153.67 peaks correspond to full-length vMIP-III and vMIP-III, which have been truncated at the carboxy terminus, respectively.$

Purification and characterization of vMIP-III expressed in CHO cells.

(A) vMIP-III was purified from culture supernatants of stably transfected CHO cells by cation exchange chromatography. Column washes were performed at 0.2 mol/L NaCl/20 mmol/L Tris, pH 7.2, and vMIP-III was eluted at 0.8 mol/L NaCl/20 mmol/L Tris. (B) SDS-PAGE analysis followed by Coomassie blue staining of the peak fractions shown in A. (C) vMIP-III was subjected to MALDI-MS. The 9677.08 and 9153.67 peaks correspond to full-length vMIP-III and vMIP-III, which have been truncated at the carboxy terminus, respectively.

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