Unique fibrinogen gene sequence variations detected in subject from Dunedin.

Abnormal bases are denoted by an asterisk. Lanes labeled N correspond to normal control sequence, while those labeled D are from propositus. Nucleotide positions are based on the numbering in the respective Genbank entries M64 982 (Aα), M64 983 (Bβ) and M10 014 (γ). (A) Sequence surrounding nucleotide 3237 in intron 4 of the Aα-chain gene obtained with the forward primer Fn3003γ (5′-TTACAGACAAATCACTCAGCAGCT-3′). Dunedin subject is heterozygous for a T→C transition. (B) Sequence surrounding nucleotide 5906 in exon 5 of the Bβ-chain gene obtained with the reverse primer Fn6050β (5′-GTATGGACATTAAGGTCGTG-3′). Dunedin subject is heterozygous for a G→A (coding strand C→T) transition, which predicts the substitution Bβ235 P→L. (C) Sequence surrounding nucleotide 8525 in the 3′ untranslated region of the Bβ-chain gene obtained with the reverse primer Fn8610β (5′-TGAACATTCCTTCCTCTTCG-3′). Dunedin subject is heterozygous for an A→C transversion. (D) Sequence surrounding nucleotide 2525 in exon 4 of the γ-chain gene obtained with forward primer Fn2479γ (5′-GGATTTTTATGTCTCTGATC-3′). Dunedin subject is heterozygous for a C→G transversion, which predicts the substitution γ82 A→G.