PS vesicles adhere to matrix TSP.

EC-depleted matrix was incubated with lipid vesicles and subsequently labeled for immunofluorescence by polyclonal antibodies to TSP and streptavidin-Texas Red. Panel A was labeled with PS vesicles; panel B was labeled with PC vesicles. Panels C and D are of the same fields labeled with both PS vesicles and TSP; C was photographed with excitation for green fluorescence to detect the phospholipid labeling, and D was photographed with excitation for red fluorescence to detect TSP.